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KMID : 0382619930130020669
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Hanyang Journal of Medicine
1993 Volume.13 No. 2 p.669 ~ p.676
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Isolation of Ovarian Tumor-associated Antigens from Ascitic Fluid of Patients with Ovarian Carcinoma
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노만택
박장환/정용훈/김정목/김경태/조양자
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Abstract
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Immune complexes were isolated from ascitic fluid of patients with ovarian carcinoma by using protein-A Sepharose CL-4B affinity chromatography, and antigen-antibody complexes then dissociated by ion exchange chromatography in the presence of 8M
urea.
Only one antigen fraction was obtained by the ion exchange chromatography, but three antigenic bands were admonstrated by SDS-PAGE. The molecular weights of the three protein bands were 70, 41, and 38 kDa, respectively. of the three proteins the
3
kDa
band has already been known, but 70 and 41 kDa bands seems to represent new ovarian tumor-associated antigens.
This study may suggest the involvement of multiple antigens in ovarian tumor which can be included in producing tumor-directed monoclonal antibodies.
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KEYWORD
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